Figure 7.

Mutagenicity test of NBD-PZ. (A and B) The umu-test bacterial strain, NM2009, was mixed with NBD-PZ (final 2.5 or 10 μg/ml), furylfuramide (0.03 μg/ml) or 2-aminoanthracene (0.3 μg/ml) in the absence (A) or presence (B) of liver homogenate, incubated at 37°C for two hours, and reacted with a chromogen. A compound is classified as mutagenic when it induces ≥ two times increase in absorbance at 670 nm compared with the sample that contains NM2009 only (the third or the first sample in A or B, respectively). Data represent the means ± standard deviations (n = 3).