Table 5. Experimental values for the blood-perfused micro-flow chamber and the cremaster muscle venule experiments and the corresponding ISWBC2 parameter values used for the two simulated experimental conditions.
Experiment | Experimental Parameter | Experimental Value | Ref | ISWBC Parameter | ISWBC Value |
I. Ex Vivo Blood Perfused Micro-flow chamber | |||||
P-Selectin | 18 µg/mL | [13] | pselectin | 15±5 | |
ICAM-1 | 15 µg/mL | [13] | icam1 | 25±5 | |
CXCL1 | 15 µg/mL | [13] | cxcl1 | 1 | |
Shear | 2.5 dyn/cm2 | [13] | RearForce | 0.6 | |
II. In Vivo Cremaster Muscle Venule | |||||
P-Selectina | 1.02×107 molecules/cm2 | [66] | pselectin | 12±5 | |
ICAM-1a | 8.90×107 molecules/cm2 | [66] | icam1 | 6±3 | |
CXCL1 | 5 µg/mL | [13] | cxcl1 | 1 | |
Shear | 1–4 dyn/cm2 | [52] | RearForce | 0.6 |
Neutrophils were incubated with radiolabelled mAbs to P-Selectin or ICAM-1 and analyzed by laser confocal microscopy. The receptor number is determined by the binding ratio between the fluorescence and immunoglobulin [66].