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. 2010 Feb 19;6(2):e1000774. doi: 10.1371/journal.ppat.1000774

Figure 5. Ats-1 is cleaved in the mitochondria.

Figure 5

A. Western blot analysis of the A. phagocytophilum- and mitochondria-containing pellet (Pellet) after 10,000×g centrifugation, and A. phagocytophilum purified from the pellet by Percoll density-gradient centrifugation [AP (Percoll)] using anti-Ats-1. Arrows 1 and 2 indicate full-length native Ats-1 and cleaved Ats-1, respectively. Molecular mass markers are indicated at left. B. Western blot analysis of A. phagocytophilum (Ap)-infected HL-60 cells and percoll-purified A. phagocytophilum [AP (Percoll)], using mouse monoclonal anti-P44 and rabbit anti-Mn-Sod. Molecular mass markers are indicated at left. C. Western blot analysis of pAts-1 or pAts-1ΔN17-transfected RF/6A cells using anti-Ats-1. Ats-1 was cleaved in A. phagocytophilum (Ap)-infected HL-60 cells and pAts-1-transfected cells, but not in pAts-1ΔN17-transfected cells. Arrows 1 and 2 indicate full-length Ats-1 and cleaved Ats-1, respectively. Molecular mass markers are indicated at left.