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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Immunol Cell Biol. 2009 Nov 17;88(2):205–212. doi: 10.1038/icb.2009.84

Figure 6. Pre-transfection of miR-146a in THP-1 cells reduces LPS-induced cytokine/chemokine production.

Figure 6

THP-1 cells were transfected with miR-146a mimic, incubated for 48 hours, and then treated with LPS for 24 hours. Culture supernatants were collected from LPS treated and untreated cells. Multiplex analysis was used to quantitatively detect cytokines and chemokines as described in Methods. The cytokine/chemokine concentrations in LPS-treated culture supernatants were normalized to untreated culture supernatants and are shown in two panels since the scales are substantially different. Mock represents level of cytokines/chemokine induced by LPS alone after 24 hours. THP-1 cells transfected with miR-146a mimic exhibited decreased production of cytokines. Average plus SD is shown, n=2 independent experiments.