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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Immunol. 2010 Jan 25;184(5):2468–2475. doi: 10.4049/jimmunol.0902450

Figure 7.

Figure 7

The effects of BU40 or recombinant CD100 on the tyrosine phosphorylation of CD72, the association with SHP-1 with CD72, and on the activation of signaling molecules in HMC1.2 cells. BU40 or recombinant CD100 administration to HMC1.2 cells up-regulates (A) the tyrosine phosphorylation of CD72, the association with SHP-1 and (B) the tyrosine phosphorylation of SHP-1. HMC1.2 cells were incubated for 0 or 5 min with control IgG, BU40, or recombinant CD100 (10 µg/ml respectively) in the absence of SCF. CD72 was immunoprecipitated with anti-CD72 (H-96) or anti-SHP-1 (C-19), and visualized with anti-phosphotyrosine 4G10, anti-SHP-1, or anti-CD72. Data are representative from three individual experiments. (C) Ligation of CD72 with BU40 or recombinant CD100 suppresses the activation of signal molecules in HMC1.2 cells. HMC1.2 cells were incubated for the indicated time with control IgG, BU40, or recombinant CD100 (10 µg/ml respectively). The levels of phospho-KIT, phospho-ERK, phospho-Src family kinases (Tyr 416), and β-actin were then assssed by immunoblot analysis. Data are representative from three individual experiments.