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. Author manuscript; available in PMC: 2011 Feb 9.
Published in final edited form as: Mol Cancer Res. 2010 Feb 9;8(2):204–215. doi: 10.1158/1541-7786.MCR-09-0253

Figure 4. Kinetic analysis of BER using a uracil-containing oligonucleotide duplex DNA substrate.

Figure 4

(B and C) Incorporation of [α-32P]dCMP was measured as a function of incubation time using various DT40 cell extracts. (B) Sequence of a 35-bp oligonucleotide containing a uracil residue. (C) Photographs of PhosphorImager analysis illustrating uracil-DNA BER are shown. (D) The relative amount of ligated uracil-BER product is represented.