Skip to main content
. 2010 Feb 19;6(2):e1000779. doi: 10.1371/journal.ppat.1000779

Figure 4. HLA-restriction and minimal optimal epitope of Gn461–475.

Figure 4

(A) Frequency of HLA-A- and B-alleles among individuals with positive response towards Gn461–475, indicating that HLA-B*35 is the only allele in common. (B) HLA-B*35-restriction of Gn461–475. Two Gn461–475–specific T-cell lines were generated over 21 days and then stimulated by Gn461–475 in the presence of autologous or heterologous APCs (B-LCL). * indicates significant responses (p<0.05). (C) downmapping of the minimal epitope of Gn461–475 Gn. Truncated versions of Gn461–475 were used to stimulate a 21-day Gn461–475–specific T-cell line (left panel) derived from a HLA-B*3502-positive donor in an 24 hour IFN-γ ELISPOT assay. Identical results were obtained with cell lines from two HLA-B*3501-positive individuals (data not shown) and in an ex vivo ELISPOT assay using PBMC from a HLA-B*3505-positive individual (right panel).