Figure 4. Id3^−/− γδ T cells are highly enriched for cells with a Vγ1.1/Vδ6.3 TCR.

A) FACS analysis of thymocytes for TCRγδ and Vγ1.1 (top panel). The frequency of TCRγδ⁺Vγ1.1⁺ cells is shown. TCRγδ⁺Vγ1.1⁺ cells were analyzed for expression of Vδ6.3 (bottom panel). The frequency of Vγ6.3⁺ cells in the TCRγδ⁺Vγ1.1⁺ population is shown. B) Frequency of Vγ1.1⁺Vδ6.3⁺ cells among TCRγδ⁺ cells in Id3 ^+/+ and Id3 ^−/− mice. Data were calculated by dividing the frequency of Vγ1.1⁺Vδ6.3⁺ cells by the frequency of TCRγδ⁺ cells (×100). Each dot represents the frequency from on mouse. The line represents the average value. C) Analysis of thymocytes for expression of TCRγδ and Vγ2, Vγ5 or Vγ3 in Id3^+/+ and Id3^−/− thymocytes. One of 4 representative experiments is shown.