Figure 4. Viability of peripheral blood mononuclear cells (PBMCs) and epithelial cell lines after culture with tenofovir gel.

PBMCs or epithelial cell lines (HEC-1-A or Caco-2) were cultured for 24 hours in dilutions of tenofovir or vehicle control gel. Dilutions were made in the appropriate medium for each cell type. Cell viability was measured using CellTiter-Glo® according to the manufacturer's instructions and was calculated as described in the Methods section. The dilution that was used for subsequent work was the lowest dilution to result in ≥60% viability of the cells. The data shown represent the mean ± standard deviation of 5 independent experiments performed in triplicate.