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Expression of genes associated with smooth muscle phenotype. Quantitative real-time PCR analysis for (A) KLF5, (B) c-myc, (C) c-fos and (D) cyclin-D obtained as described in Materials and methods. Data are presented as average fold change ± SEM from control normalised to 18S RNA. (Arterial pressure, n = 7 for KLF5 and 5 for the cell cycle genes, venous pressure n = 4.) Open bars: arterial pressure, solid bars: venous pressure. (∗p < 0.05, ∗∗p < 0.01 compared to t = 0.)
