Fig. 7.

Detection of Ag and pMHC complexes in tissue sections of pDNA-immunised mice. At different times after injection of pCI-EαRFP, pCI-EαGFP or pCIneo, we collected and processed TA muscle and draining and distal lymph nodes for immunofluorescence microscopy. GFP expression was observed at the muscle injection site, 24 h after pDNA injection (A and B). GFP⁺ muscle cells were predominantly found in the vicinity of the injection site, as evidenced by the inflammatory infiltrate at the needle trajectory (B). DAPI was used in (A) and (B) to stain cell nuclei. (C) Using anti-GFP IgG, we observed small clusters of GFP protein in draining popliteal LNs at 24 h post-injection (red). (D) We observed Y-Ae⁺ cells in the subcapsular sinus of EαRFP protein-immunised mice at 24 h (blue) and (E and F) in pCI-EαRFP-immunised mice at d3 post-plasmid injection (blue). No staining was observed in pCIneo-immunised mice or using the isotype control, mIgG2b (data not shown). Scale bars show 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)