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. Author manuscript; available in PMC: 2010 Feb 19.
Published in final edited form as: Immunity. 2009 Jul 2;31(1):25–34. doi: 10.1016/j.immuni.2009.05.008

Fig. 2. RIG-I activation requires a short double strand and prefers 5′-adenosine.

Fig. 2

Purified monocytes were stimulated with the indicated single-stranded or double-stranded synthetic RNA oligonucleotide. IFN-α production was analysed 24 hours after stimulation. Data from n independent donors are depicted as mean values ± SEM, n as indicated. A: 3P-A was hybridized with synthetic antisense single-stranded RNA with different lengths generating double-stranded RNA with blunt end carrying the triphosphate group. B: 23mer (AS G23 and AS A23) with a blunt triphosphate end and a 3′overhang at the non-triphosphate end. C: Comparison of RIG-I ligand activity of 3P-G to the other three synthetic variants (3P-A, 3P-C, 3P-U) which were hybridized with the corresponding synthetic 24mer (AS-A24, AS-C24, AS-U24) or used as single strands. D: IFN-α inducing activity of 3P-A+AS A24 and 3P-G+AS G24.