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. Author manuscript; available in PMC: 2010 Feb 19.
Published in final edited form as: J Immunol. 2009 Jul 1;183(2):787–791. doi: 10.4049/jimmunol.0901363

Figure 2. PRR activation is required for NLRP3 inflammasome activation.

Figure 2

(A) Immunoblot of caspase-1 in wild type or knock-out macrophages primed for 4h with LPS (200 ng/ml), Pam2CysK4 (50 ng/ml), TLR4 activating (UT18), or TLR4 blocking (MTS510) antibodies and subsequently stimulated with ATP (1h). Cells from wild-type or knock-out macrophages were left untreated or stimulated for 4h with (B) poly(I:C) (1 µg/ml), Pam2CysK4 (50 ng/ml), or R848 (0.5 µg/ml) or with (C) Pam2CysK4 (50 ng/ml), MDP (10 µg/ml), LPS (200 ng/ml), or transfected with poly(dA-dT). ATP was added for 1h as indicated. (D) Wild type or TNFR1/2 dKO macrophages were stimulated with TNFα as indicated or with LPS as in (A) and analyzed for caspase-1 activation. Immunoblot analyses of cleaved caspase-1 from supernatants are show. Data are from one representative experiment out of three (A, B) or two (C–D).