Figure 3. IL-1β responses to Candida albicans are mediated by NLRP3, ASC and Caspase-1.
(a) IL-1β responses of macrophages from Asc-/-, Nlrp3-/- and wild-type mice primed for 4 h with 500 ng/ml LPS then stimulated with C. albicans at ~ 106/ml; (b) Western blot of supernatants from wild-type or Asc-/- macrophages probed with anti-IL-1β antibody; (c) IL-1β responses of macrophages from Casp1-/- and wild-type mice stimulated overnight with C. albicans at ~ 106/ml; (d) IL-1β responses of macrophages from P2X7R-/- and wild-type mice stimulated overnight with C. albicans at ~ 106/ml. (*** P < 0.001; ** P < 0.01; * P < 0.05)