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Effect of thiopental (50 µM) on mitochondrial membrane potential in hypoxia-reoxygenated cells. Mitochondrial membrane potential was estimated by flow cytometry. Cells were labeled with JC-1 for 20 min at 37℃ (n = 5). Hypoxia-reoxygenation increased mitochondrial membrane depolarization (middle panel), and thiopental treatment inhibited this effect (right panel). Control: normal cardiomyocytes. H/R: hypoxia-reoxygenated cardiomyocytes.
