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Time-dependent changes in anomer composition of streptozotocin solutions. Streptozotocin was dissolved in citrate buffer and kept at room temperature in the dark. Samples were injected at the indicated intervals for measuring α and β anomers by HPLC. Equilibrium between anomers (50:50) was achieved 1 h after dissolution (upper graph). Equilibration of the solution at a ratio of 44% α: 56% β was reached after approximately 2 h (upper and lower graphs).
