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Effects of shear stress (15 dyn/cm2) and endothelin-1 (ET-1; 0.01 μM) on endothelin B (ETB) receptor expression in rat pulmonary microvascular endothelial cells (RPMVECs). Top shows representative RT-PCR for the ETB receptor mRNA (12 h) and immunoblots for ETB receptor protein (24 h). Bottom summarizes normalized ETB receptor mRNA and protein levels. Values are expressed as means ± SE (n = 5–7 for each group). *P < 0.05 compared with control cells.
