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Polyplex disassembly rate, measured by particle volume decrease with time, as a function of the N/P ratio and salt concentration. The particle volume was determined using the bearing analysis of the AFM images. Time zero corresponds to the addition of 20 mM DTT. (a) Disassembly rate as a function of the N/P ratio (salt concentration = 0.4 M NaCl). (b) Disassembly rate as a function of the salt concentration (N/P = 4).
