Figure 6.

TRPM7-mediated cell rounding is independent of the divalent cation permeated by the channel. (a) Cell rounding analysis of 293-TRPM7 expressing cells (+TET) after 24 hours of culture in regular DMEM media, which contained 0.8 mM Mg²⁺/ 2 mM Ca²⁺, or regular DMEM media with a total of 10 mM Mg²⁺, 25 mM Mg²⁺ or 5 mM Ca²⁺. (b) Quantification of the degree of cell rounding under the conditions depicted in (a). (c) Calpain activity assay of 293-TRPM7 expressing cells (+TET) under the conditions described in (a). Scale bar is equal to 100 μm. The experiments were repeated three times with similar results.