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. Author manuscript; available in PMC: 2011 Mar 5.
Published in final edited form as: J Mol Biol. 2010 Jan 11;396(4):858–869. doi: 10.1016/j.jmb.2010.01.014

Figure 7.

Figure 7

Overexpression of TRPM7 elevates cellular Mg2+ levels. (a) Measurement of intracellular free Mg2+ in 293-TRPM7 cells. Cells were loaded with the fluorescent ratiometric indicator Mag-Indo-1. Baseline Mag-Indo violet to Mag-Indo blue ratios were measured using a Beckman Coulter MoFlo XDP cell sorter. Fluorescence emission ratios were displayed using a histogram. Overexpression of TRPM7 (+TET) caused an increase in intracellular free Mg2+ compared to control cells (293-TRPM7-TET). Data are representative of five independent experiments. (b) ICP-MS quantitation of total Mg2+ in HNO3 extracts of 293-TRPM7 cells with and without tetracycline treatment, where average concentration of total cellular Mg2+ was calculated by normalizing to a [K+] of 120 mM. Values are mean ± standard deviation of at least two independent experiments. The asterisk indicates a significant difference in total Mg2+ compared to the negative control (293-TRPM7-TET) using a Student t test (p<0.05).