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Effect of sceptrin on cell motility in different cancer cell lines. a−c) Indicated cells were seeded on top of fluorescence beads and treated with sceptrin (40 μM). Basal random motility was measured for cells in panels a and b. Motility was stimulated by hepatocyte growth factor (HGF) (10 ng mL−1) in panel c. Cells were fixed after 24 h, a picture of each well (all the conditions were assayed in triplicate) was taken, and the surface of the cell tracks was segmented and quantified.
