Figure 3.

Effect of sceptrin on apoptosis and cell proliferation. a) Effect of sceptrin on cell proliferation was tested in HeLa cells, which were seeded at low density and treated with vehicle, sceptrin (40 μM), or positive control aphidicolin (5 nM). Proliferation was quantified by cell counting at indicated times. b) Putative induction of apoptosis by sceptrin was assayed in HeLa cells, which were treated with sceptrin at indicated concentrations or with the positive control staurosporine (5 nM) for 24 h. Apoptosis was quantified by immunochemical determination of histone-complexed DNA fragments in a microplate well.