Figure 6.

Time-lapse confocal video microscopy of cells treated with sceptrin. a) Effect of sceptrin on cell motility dynamics was tested on MDA-MB-231 cells transiently transfected with GFP-actin construct. Cells that had been seeded on fibronectin-coated LabTek chambers were pictured (20x objective) every 2 min up to 3 h. Panel a shows an RGB composite still image taken at time 0, 1, 2, and 3 h, where red represents 0 h, green 1 h, blue 2 h and white 3 h. Actual movies can be seen in online Supporting Information. b) Effect of sceptrin on HeLa cells was assayed as described for panel a. HeLa cells were induced to migrate by addition of hepatocyte growth factor (HGF) (10 ng mL⁻¹). A 40x objective was used to take the pictures in this case.