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. Author manuscript; available in PMC: 2010 Feb 19.
Published in final edited form as: J Gene Med. 2006 Jan;8(1):18–28. doi: 10.1002/jgm.825

Figure 3.

Figure 3

Effect of time-in-culture on the susceptibility of primary human monocytes to DLV transduction. Adherent monocytes were grown in RPMI supplemented with 10% human serum and 20% FBS and transduced with DLV (MOI = 10) at the indicated times. Transduction efficiency was determined by calculating the percentage of GFP+ cells within the culture. Transduction efficiency increased significantly from day 3 to day 5 (+p < 0.001) and from day 5 to day 7 (++p < 0.01), but there was no significant increase after day 7 (*p > 0.05) using one-way ANOVA followed by Bonferroni's multiple comparison test