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. 2010 Feb 10;30(6):2165–2176. doi: 10.1523/JNEUROSCI.5123-09.2010

Figure 2.

Figure 2.

Interneurons expressing nNOS belong to an MGE lineage. A, Histogram showing the distribution of GFP-positive cells in CA1 of Nkx2-1BAC-Cre/RCE:LoxP mice (n = 1992). B, C, Images illustrating the coexpression between GFP, PV, and nNOS. Filled arrowheads indicate interneurons expressing PV (B) or nNOS (C) and GFP. Transparent arrowheads point to PV (or nNOS)-positive interneurons that are negative for GFP. D, In green, the number of Nkx2-1-derived cells coexpressing GFP and nNOS presented as a percentage of the total number of GFP+ cells. In red, the contribution of Nkx2-1-expressing precursors to the population of nNOS+ interneurons in CA1 (n = 990). E, F, Images illustrating the low coexpression between CoupTFII with GFP and nNOS. G, Fraction of interneurons coexpressing CoupTFII and nNOS presented as a percentage of the total number of nNOS+ cells (n = 695). H, Neurolucida reconstruction of a NGC GFP+ interneuron. Scale bar: 50 μm. I, Voltage responses of cell shown in H to three current step injections (−200 pA, just suprathreshold, and twice the suprathreshold current). J, Phase plot of the voltage response shown in H to the 2× suprathreshold current. Inset illustrates overlaid action potentials; scale bar: 10 mV, 2 ms. K, Single-cell RT-PCR analysis of the cell shown in H. The numbers under the gel represent the summary data for all tested GFP+ interneurons recorded in s.l.m. Scale bars: 50 μm.