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. 2009 Oct 14;82(3):516–527. doi: 10.1095/biolreprod.109.080374

FIG. 5.

FIG. 5.

SB-3CT administration causes the changes in expression levels of junction proteins in vivo and in vitro. In vivo, 28-day-old C57BL/6J mice are pretreated with SB-3CT (0, 5, 10, and 25 mg/kg) for 6 h and then posttreated with 1 g/kg of MEHP for another 12 h. In vitro, primary rat Sertoli cell-germ cell cocultures are treated with SB-3CT (0, 5, and 10 μM) in the presence of MEHP for 12 h. The protein levels of claudin11, occludin, ZO1, laminin-γ3, and β1-integrin in whole testis homogenates (A) and in primary rat Sertoli cell-germ cell cocultures (C) are determined by Western blot analysis. ACTB serves as the loading control. B) The quantified relative protein levels of A. D) The quantified relative protein levels of C. Values represent the mean ± SEM. Asterisks denote significant differences between the treatments and the control (*P < 0.05, Student t-test).