Table 1.
Percentage of positive phage peptides identified by rAb 2B4 and 3B using different culture conditionsa.
| Second pan | Third pan | Fourth pan | |
|---|---|---|---|
| rAb 2B4 | |||
| Condition 1 | 6.25% | 0 | 4.50% |
| Condition 2 | 12.50% | 18.75% | 18.75% |
| rAb 3B | |||
| Condition 1 | 45.80% | 20.80% | 21% |
| Condition 2 | 37.50% | 62.50% | 68.75% |
a
After each pan, eluted phage were plated and phage plaques were amplified in U96-Deepwell plates. Phage were considered positive when the ELISA OD value was at least three times that of the negative control. Condition 1: 250 μl of E. coli cells used for amplification; elution at room temperature for 20 min. Condition 2: 150 μl of cells used for amplification; elution at 37 °C for 10 min.