Table 2.
Cycle threshold (Ct) values of candidate EC genes and PCDC4 in colorectal tissues
| EC Gene | Ct Range | Ct Min | Ct Max | Mean Ct ± s.e.m | Standard deviation (SD) |
|---|---|---|---|---|---|
| B2M | 4.03 | 17.47 | 21.51 | 19.48 ± 0.14 | 1.04 |
| PPIA | 4.13 | 17.78 | 21.91 | 19.90 ± 0.14 | 1.06 |
| GAPDH | 5.80 | 18.51 | 24.32 | 21.00 ± 0.17 | 1.29 |
| ACTB | 12.74 | 21.08 | 33.32 | 25.14 ± 0.34 | 2.61 |
| HPRT | 8.54 | 22.74 | 31.28 | 26.68 ± 0.25 | 1.89 |
| DTX3 | 6.6 | 24.95 | 31.56 | 28.62 ±0.17 | 1.37 |
| SLC25A23 | 7.26 | 24.48 | 31.74 | 27.36 ±0.19 | 1.54 |
| CHRNB4 | 9.40 | 27.99 | 37.38 | 32.30 ±0.19 | 2.15 |
| RTDR1 | - | 30.59 | UD | 35.82 ±0.36 | 2.15 |
| HCRT | - | 33.96 | UD | 38.46 ±0.29 | 1.67 |
| APOC4 | - | UD | UD | - | - |
| KRTAP12-3 | - | 33.16 | UD | 36.95 ±0.19 | 1.46 |
| MRPL19 | 4.10 | 26.70 | 30.80 | 28.62 ± 0.13 | 0.98 |
| CXCL12 | 13.54 | 21.85 | 35.39 | 25.77 ± 0.32 | 2.61 |
| FABP1 | 15.57 | 16.61 | 32.19 | 20.83 ± 0.40 | 3.24 |
| MUC2 | 17.71 | 17.62 | 35.33 | 22.43 ± 0.53 | 4.16 |
| PDCD4 | 11.92 | 21.35 | 33.27 | 24.56 ± 0.32 | 2.59 |
B2M and PPIA were the most abundantly expressed genes, having the lowest mean Ct values while MRPL19 was the least abundantly expressed gene with average Ct values > 26. Both B2M and PPIA had the lowest range in their Ct values.