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. 2010 Feb 1;7:24. doi: 10.1186/1743-422X-7-24

Figure 5.

Figure 5

Clathrin-mediated endocytosis of DENV into Huh7 cells. Huh7 cells treated with (A) chlorpromazine shows marked reduction in the infectious entry of DENV, whereas (B) filipin does not significantly inhibit virus entry. The solvent (MET, methanol) control for filipin treatment is also included. Minimal cytotoxicity was observed for the concentration range of chlorpromazine and filipin used in this assay. The average of three independent experiments is shown. (C) Inhibition of DENV entry into Huh7 cells expressing EPS15 dominant-negative mutant protein. The infectious entry of DENV is significantly inhibited in Huh7 cells transfected with GFP-EPSΔ95/295 when compared to mock-transfected or pEGFP transfected cells. The number of viral E antigen-positive cells in relation to the total cell population is expressed as a percentage of viral antigen-positive cells. The average of three independent experiments is shown. (D) DENV (stained with TR failed to infect GFP-EΔ95/295 expressing cell. In contrast, internalized DENV particles (arrows) are observed within cells expressing the negative control plasmid (EGFP-C2) expressing GFP.