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Oligonucleotide primers used for Marek's disease virus PCR analysis
| UL49amp_fw | 5'-ATGGCGACGCGAAGTTG-3' |
|---|---|
| UL49amp_rv | 5'-CCATCAACACGTACTCAGCGA-3' |
| UL49disc_fw | 5'-CTTGTACGTTCAGATTTGGTTG-3' |
| UL49disc_rv | 5'-TCATCAGCATCTAGCACTTGG-3' |
Letters in bold indicate three nucleotides at the 3'-end of UL49disc_fw that mismatch with the UL49-sequence of MDV-CVI988/Rispens (...ACT-3').
