Table 3.
Effect of scrotal heat stress and γ-radiation on Tail length on Comets from spermatozoa recovered after in vitro fertilization from zona pellucida and assessed by neutral Comet assay
| Treatment | Tail Length | Tail Moment | ||
|---|---|---|---|---|
| IVF | ZP† | IVF | ZP† | |
| Control | 16.20 ± 1.02aα | 14.76 ± 3.98a | 4.55 ± 0.56aα | 3.72 ± 1.30a |
| (n = 9) | ||||
| Scrotal Heat Stress | 26.64 ± 3.34aβ | 15.96 ± 2.92b | 14.82 ± 2.34aβ | 8.96 ± 3.31b |
| (n = 9) | ||||
| γ-Radiation | 46.95 ± 6.55aγ | 21.43 ± 5.84b | 29.90 ± 6.34aγ | 8.68 ± 3.15b |
| (n = 8) | ||||
Significant differences in tail length and tail moment from spermatozoa recovered in each step of IVF, within a treatment, were denoted with different Latin alphabetical superscript in each row; and within an IVF step were denoted with different Greek superscripts in each column. Means ± SEM. Values with different superscripts differ significantly (P < 0.05) Ep, epididymal sperm; IVF, sperm population recovered from in vitro fertilization drop; ZP, sperm population attached to Zona Pellucida. † Indicates no significant differences among treatments within ZP.