Table 4.
Cleavage rate and development of oocytes fertilized by In Vitro fertilization and ICSI with non-treated, scrotal heat stressed and γ-radiated spermatozoa
| Technique | Sperm treatment | Total oocytes (experiments) | 2-cell embryos (%) | Blastocyst (%) | |
|---|---|---|---|---|---|
| IVF | Control | 107 (5) | 99 (93)a | 95 (96)a | |
| Scrotal Heat Stress | 223 (8) | 29 (13)b | 26 (90)a | ||
| γ-Radiation | 85 (5) | 56 (66)c | 36 (63)b | ||
| Sperm treatment | Injected Oocytes (experiments) | Surviving Oocytes (%) |
2-cell embryos (%) |
Blastocyst (%) |
|
| ICSI | Control | 72 (3) | 53 (74) | 46 (87)a | 31 (67)a |
| Scrotal Heat Stress | 232 (6) | 158 (68) | 74 (47)b | 4 (6)b | |
| γ-Radiation | 131 (4) | 94 (72) | 65 (69)c | 28 (43)c |
Means ± SEM. Different alphabetical superscript indicates significant differences (P < 0.05, ANOVA analysis) among treatments.