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. 2010 Jan 26;9:15. doi: 10.1186/1476-4598-9-15

Figure 5.

Figure 5

9-cis-RA pretreatment prevents apoptosis induced by cancer chemotherapy insults in T47D cells, but increases chemotherapy-induced apoptosis in H3396 cells: correlation with NF-κB activity induction by 9-cis-RA. (A) EMSA performed with probes bearing the NF-κB-1 and NF-κB-3 sites of the cIAP2 promoter and nuclear extracts from T47D and H3396 cells treated with 1 μM 9-cis-RA for different times, as indicated. The images shown are from one representative experiment performed twice with similar results. (B) T47D and H3396 cells were pretreated with or without 9-cis-RA for 30 h, followed by treatment with different doses of the indicated chemotherapeutic agents for 72 h and death ligands for 48 h. Apoptotic cells were determined by FACS analysis after staining with propidium iodide as described in "Materials and Methods". The values represent the mean ± SD of three independent experiments performed in duplicate. Asterisks denote statistically significant differences between untreated cells and cells treated with 9-cis-RA.