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. 2010 Feb 22;5(2):e9344. doi: 10.1371/journal.pone.0009344

Figure 2. Analyses of protein interactions by FRET.

Figure 2

(a) Representative primary FACS-plots showing the amount of FRET+ cells in living 293T cells cotransfected with the indicated CFP and YFP fusion proteins. Numbers give total percentages of cells within the FRET gate (compare Fig. 1a panel 3). (b) Mean values and standard deviations (SD) for the total amount of FRET+ cells from a minimum of eight independent experiments that were analysed as depicted in (a). The dotted line gives the maximum background FRET-signal that was obtained when cells were cotransfected with the MEM-CFP control and the YFP fusion proteins. Abbreviations: CfY, CFP fused YFP; CaY, CFPonly and YFP-fusion. (c) Merged images from confocal pictures of 293T cells that were cotransfected with the Nef/Vpu-YFP fusion proteins (shown in green) and the indicated CFP-fusions (shown in red). Regions in which both fusions colocalize appear yellow.