Skip to main content
. 2010 Feb 22;5(2):e9342. doi: 10.1371/journal.pone.0009342

Figure 4. Electroporation and microinjection of αH6 induces apoptosis in Jurkat cells.

Figure 4

Jurkat cells were electroporated with 2.5 µM of αH6-FITC and αH6m-FITC peptides, as described previously (Gabriel 2003). The efficiency of peptides electroporation into these cells was determined by measuring the FITC fluorescence using flow cytometry (A). Mitochondria were isolated from these cells, and analyze by flow cytometry described previously [36]. (B and C) Effect of αH6 and αH6m electroporation (2.5 µM) on mitochondrial potential, caspases 3/7 activation and cell viability in Jurkat cells. (B) Left panels: dot plot of DIOC(6)3 vs PI staining cells. Right panels: caspases 3/7 activities by cleavage of the Phiphilux substrate. E refers to electroporated cells. (C) Left pannels: dose response of αH6 and αH6m on mitochondrial potential, caspases 3/7 activation and apoptosis. Right panels: Caspase-3 and PARP cleavage were analyzed by Western Blotting. (D) Dose response curves of caspases 3/7 activation induced by electropermeabilization of tBid, αH6-BH3, and αH6 in Jurkat cells. (E) Jurkat cells were microinjected with tBid, αH6 and αH6m and the percentage of cell blebbing was measured by microscopy (F). Data are representative of 10 independant measurements (n = 10).