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. 2010 Feb 22;5(2):e9354. doi: 10.1371/journal.pone.0009354

Figure 3. Regulation of GFP expression in M. smegmatis, BCG, and Mtb H37Ra by unnatural amino acid mediated nonsense codon suppression.

Figure 3

A. pIpa-GFP151TAG-M. smegmatis in 96 well flat-bottom plates was incubated with the indicated concentration of pIpa for 16 hrs. Representative phase contrast and GFP fluorescence images for each condition are shown; B. pIpa-GFP151TAG-M. smegmatis, pIpa-GFP151TAG-BCG, and pIpa-GFP151TAG-H37Ra were incubated in the absence (open histograms) or presence (filled historgrams) of 1 mM pIpa for 16 hrs. The GFP fluorescent intensity of the bacteria populations was determined by flow cytometry.