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Release of intact Myo1-GFP rings from S. cerevisiae cells. A & B) Isolated Myo1-GFP rings from S. cerevisiae. Rings were concentrated for visualization by 13,000xg pelleting (See Figure 2). A) Comparisions of long pass GFP (GFPLP) fluorescent images and bright field images of close-up views of rings. Bright field images show that rings are not associated with cells. B) whole camera field views of rings. Bars ∼ 1 μm (1032 nm).
