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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Jan;67(1):32–42. doi: 10.1002/cm.20419

Figure 3.

Figure 3

Two sets of siRNAs each were used to knock down the expression of myosin IIA (IIA1 and IIA2) and IIB (IIB1 and IIB2) specifically. A non-silencing siRNA was transfected to use as a control (CTL). (A, B) The levels of myosin IIA and IIB proteins were analyzed at 72 hours after transfection of siRNA by immunofluorescent staining using antibodies against myosin IIA (A) and myosin IIB (B). (C) Decrease of myosin II proteins in the cells transfected with the siRNAs was also confirmed by western blot of cell extracts at 72 hours post-transfection. An antibody against β-actin was used as loading control. Scale bar=10 μm.