Fig. 1.

Direction of mechanical loading in the tibia/fibula and representative transverse μCT images at the analyzed sites in the tibia, fibula, femur, ulna and radius of a 21-week-old female C57BL/6 mouse. In the tibia, proximal (25% of the bone's length from its proximal end), proximal/middle (37%), middle (50%) and distal (75%) sites in cortical bone and two sites 0.01–0.25 mm (containing primary spongiosa) and 0.25–1.25 mm (secondary spongiosa) distal to growth plate in trabecular bone were analyzed. In the fibula, femur, ulna and radius, the middle (50%) sites in cortical bones were analyzed.