Table 3.
Cortical parameters analyzed by μCT and histomorphometry in the tibia of 21-week-old female C57BL/6 mice that received no artificial loading, static (2.0 N) loading or static (2.0 N) plus dynamic (11.5 N) loading in the right tibia/fibula for 2 weeks.
| NOLOAD left | NOLOAD right | STATIC left | STATIC right | DYNAMIC + STATIC left | DYNAMIC + STATIC right | |
|---|---|---|---|---|---|---|
| Proximal | ||||||
| Periosteally enclosed volume (mm3) | 0.719 ± 0.012 | 0.711 ± 0.009 | 0.715 ± 0.005 | 0.717 ± 0.010 | 0.720 ± 0.013 | 0.832 ± 0.007⁎ |
| Bone volume (mm3) | 0.418 ± 0.006 | 0.411 ± 0.004 | 0.412 ± 0.003 | 0.411 ± 0.005 | 0.403 ± 0.008 | 0.548 ± 0.013⁎ |
| Medullary volume (mm3) | 0.301 ± 0.007 | 0.300 ± 0.007 | 0.303 ± 0.004 | 0.306 ± 0.009 | 0.316 ± 0.009 | 0.284 ± 0.009⁎ |
| Periosteal newly formed bone area (%) | 7.7 ± 1.1 | 9.5 ± 1.3 | 8.5 ± 1.5 | 10.0 ± 1.6 | 10.6 ± 1.6 | 62.1 ± 2.7⁎ |
| Endosteal newly formed bone area (%) | 10.0 ± 0.6 | 9.8 ± 0.9 | 8.3 ± 1.0 | 9.9 ± 0.8 | 9.0 ± 1.4 | 22.8 ± 1.1⁎ |
| Proximal/middle | ||||||
| Periosteally enclosed volume (mm3) | 0.609 ± 0.006 | 0.621 ± 0.005 | 0.620 ± 0.007 | 0.623 ± 0.011 | 0.624 ± 0.011 | 0.742 ± 0.004⁎ |
| Bone volume (mm3) | 0.382 ± 0.004 | 0.383 ± 0.003 | 0.382 ± 0.002 | 0.384 ± 0.006 | 0.378 ± 0.008 | 0.501 ± 0.006⁎ |
| Medullary volume (mm3) | 0.227 ± 0.005 | 0.238 ± 0.006 | 0.237 ± 0.007 | 0.238 ± 0.006 | 0.246 ± 0.006 | 0.240 ± 0.005 |
| Periosteal newly formed bone area (%) | 4.6 ± 0.4 | 6.4 ± 1.0 | 5.4 ± 0.6 | 6.0 ± 0.7 | 6.5 ± 0.5 | 64.3 ± 1.8⁎ |
| Endosteal newly formed bone area (%) | 9.4 ± 0.7 | 9.2 ± 1.1 | 7.9 ± 1.4 | 6.5 ± 1.3 | 7.2 ± 0.6 | 22.8 ± 0.9⁎ |
| Middle | ||||||
| Periosteally enclosed volume (mm3) | 0.504 ± 0.006 | 0.499 ± 0.006 | 0.508 ± 0.007 | 0.507 ± 0.008 | 0.506 ± 0.012 | 0.573 ± 0.011⁎ |
| Bone volume (mm3) | 0.307 ± 0.003 | 0.305 ± 0.001 | 0.309 ± 0.002 | 0.310 ± 0.003 | 0.310 ± 0.006 | 0.384 ± 0.007⁎ |
| Medullary volume (mm3) | 0.197 ± 0.004 | 0.194 ± 0.006 | 0.199 ± 0.005 | 0.197 ± 0.006 | 0.196 ± 0.008 | 0.189 ± 0.006 |
| Periosteal newly formed bone area (%) | 6.9 ± 0.8 | 12.8 ± 2.3 | 9.3 ± 1.8 | 6.8 ± 1.3 | 9.7 ± 1.2 | 44.5 ± 0.9⁎ |
| Endosteal newly formed bone area (%) | 5.5 ± 1.0 | 5.7 ± 0.9 | 3.8 ± 0.9 | 5.0 ± 1.2 | 3.2 ± 0.7 | 18.3 ± 1.1⁎ |
| Distal | ||||||
| Periosteally enclosed volume (mm3) | 0.385 ± 0.004 | 0.378 ± 0.005 | 0.382 ± 0.005 | 0.379 ± 0.008 | 0.385 ± 0.006 | 0.389 ± 0.007 |
| Bone volume (mm3) | 0.281 ± 0.003 | 0.273 ± 0.003 | 0.278 ± 0.003 | 0.274 ± 0.005 | 0.275 ± 0.004 | 0.270 ± 0.004 |
| Medullary volume (mm3) | 0.103 ± 0.003 | 0.104 ± 0.003 | 0.104 ± 0.003 | 0.105 ± 0.003 | 0.110 ± 0.005 | 0.119 ± 0.006 |
| Periosteal newly formed bone area (%) | 8.9 ± 0.7 | 8.4 ± 1.0 | 9.2 ± 2.8 | 10.5 ± 1.6 | 9.7 ± 1.1 | 13.9 ± 0.8⁎ |
| Endosteal newly formed bone area (%) | 6.5 ± 1.3 | 7.0 ± 0.7 | 5.3 ± 0.9 | 7.9 ± 1.8 | 6.0 ± 1.0 | 7.1 ± 1.0 |
Mean ± SE (n = 6–7).
⁎
p < 0.05 versus left in the DYNAMIC + STATIC group by paired t-test.