Fig. 3.

No effect of cell culture oxygen levels on the viability of proliferating myoblasts. For all graphs, 5% oxygen culture conditions are represented by black bars and 20% oxygen culture conditions are represented by white bars. a Cell viability, as determined by Trypan blue staining, was similar in proliferating myoblasts cultured in 5 or 20% oxygen at 24 h, 3 and 6 days post-seeding. b Indicative of the increase in myoblast numbers in low oxygen culture conditions, LDH activity was greater in the cell lysate and medium from 5% oxygen compared to 20% oxygen cultures (p < 0.05; paired t-test). c In concordance with the Trypan blue data, when medium and cell lysate LDH activity were expressed as a viability ratio, oxygen concentration had no significant effect on the viability of proliferating myoblasts