Table 1.
Oligonucleotide sequences for real-time PCR
| Gene amplified | Oligonucleotide sequence 5′ → 3′ | Chemistry |
|---|---|---|
| Myogenin | For: GGTGCCCAGCGAATGC | SYBR |
| Rev: TGATGCTGTCCACGATCGA | ||
| MyoD | For: CCGCCTGAGCAAAGTAAATGA | SYBR |
| Rev: GCAACCGCTGGTTTGGATT | ||
| MyHC IIa | For: AAGGTCGGCAATGAGTATGTCA | SYBR |
| Rev: CAACCATCCACAGGAACATCTTC | ||
| Creatine Kinase | For: GGCATCTGGCACAATGAC | SYBR |
| Rev: GATGACCCGGAGGTGATC | ||
| Cyclin A2 | For: CGTGGACTGGTTAGTTGA | SYBR |
| Rev: ATGGCAAATACTTGAGGT | ||
| CDK6 | For: TGATGTGTGCACAGTGTCACGAAC | SYBR |
| Rev: CTGTATTCAGCTCCGAGGTGTTCT | ||
| p27Kip1 | For: GCGCAGGAGAGCCAGGAT | SYBR |
| Rev: TTGGGGAACCGTCTGAAACA | ||
| P21Cip1 | For: NA | TaqMan |
| Rev: NA | (Hs99999142) | |
| PPARγ | For: NA | TaqMan |
| Rev: NA | (Hs01115513) | |
| Cyclophilin | For: CATCTGCACTGCCAAGACTGA | SYBR |
| Rev: TTCATGCCTTCTTTCACTTTGC |