Fig. 1.

Comparison of overall protein patterns of membranous and cytosolic fractions. Extracted cytosolic (Cyt) and membranous (Mem) fractions of colon cancer cells separated using SDS–PAGE and visualized by Coomassie staining in order to show overall differences in protein patterns between both fractions. Kit V yielded two cytosolic fractions (C1, C2) due to repeated separation steps. Cyt cytosolic fraction, Mem membranous fraction, vs versus. The membranous fractions of kits I, III and V showed different protein bands as compared to their corresponding cytosolic fraction. In contrast, hardly any differences in the overall expression patterns could be detected between the sub-cellular fractions of kit II and IV. Reproducibility of replicates was between 67% (Kit I) and 100% (Kit II, III, IV and V)