Table 1.
Gene name | Primer sequences (5’-3’) | |
IL-1β | Sense | GCAACTGTTCCTGAACTCAACT |
Antisense | ATCTTTTGGGGTCCGTCAACT | |
IL-6 | Sense | CCACTTCACAAGTCGGAGGCTTA |
Antisense | GCAAGTGCATCATCGTTGTTCATAC | |
IFN-γ | Sense | TCAAGTGGCATAGATGTGGAAGAA |
Antisense | TGGCTCTGCAGGATTTTCATG | |
TNF | Sense | CCACCACGCTCTTCTGTCTAC |
Antisense | TGGGCTACAGGCTTGTCACT | |
β-actin | Sense | CTAGGCACCAGGGTGTGAT |
Antisense | TGCCAGATCTTCTCCATGTC | |
miRNA155 | Stem-loop primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCCCT |
Sense | GCCCGCTTAATGCTAATTGTGAT | |
Antisense | GTGCAGGGTCCGAGGT | |
U6 | Sense | CTCGCTTCGGCAGCACA |
Antisense | AACGCTTCACGAATTTGCGT |
RT: Reverse transcription; PCR: Polymerase chain reaction.