Figure 3.

Relative expression of mRNA for P2Y₂ receptors (A), P2Y₆ receptors (B) and the ratio P2Y₆/P2Y₂ (C) in naïve murine aortic SMCs (SMC aorta), in a primary culture of aortic SMCs (cultured SMC) and in J774 macrophages (J774). The expression of P2Y₆ receptors decreased upon cultivation of SMCs, whereas J774 macrophages displayed a relatively high expression. ΔC_T= C_Tβ-actin – C_T P2Y_{2 or 6}. Results show mean and SEM, n= 5; *P < 0.05, **P < 0.01, anova, Bonferroni post hoc test. SMC, smooth muscle cell.