Table 1.
Sequences of primers and probes for real-time quantitative RT-PCR
| Gene | Sequence (from 5′ to 3′) | Amplicon |
|---|---|---|
| P2Y1 | ||
| Forward | CAAGCAGAATGG AGACACGAGT | |
| Reverse | GCATAGATCATCTCAGGG ATGTCTT | |
| Probe | ACTCAGGAGCTAGGATCTCGTGCC TTCAC | 93 bp |
| P2Y2 | ||
| Forward | ACCTCAAGAGCAGGAGCTGATC | |
| Reverse | GCCATTGATGGTGCTATTCCA | |
| Probe | CTGCTGCCATTGCCCTGGACCT | 75 bp |
| P2Y4 | ||
| Forward | GGCCAGAAGAAGCAGCAGAA | |
| Reverse | GGCTGGGACCTAGTGATGTGA | |
| Probe | CCCAGCTTCCTTAGTCCAGTCCAGGG | 145 bp |
| P2Y6 | ||
| Forward | AGCAAGGCGGCTCGTATG | |
| Reverse | TCTCCAGCACAGGGCAAGA | |
| Probe | CCTACTTGGCTGTGCGCTCCACG | 129 bp |
| β-actin | ||
| Forward | GCTCTGGCTCCTAGCACCAT | |
| Reverse | GCCACCGATCCACACAGAGT | |
| Probe | ATCAAGATCATTGCTCCTCCTGAGCGC | 75 bp |
| CD68 | Mm00839636_g1 | Assay on demand (Applied Biosystems) |
| α-SM-actin | Mm01204962_gh | Assay on demand (Applied Biosystems) |
| iNOS | Mm01309897_m1 | Assay on demand (Applied Biosystems) |
| IL-6 | Mm01210733_m1 | Assay on demand (Applied Biosystems) |
IL-6, interleukin 6; iNOS, inducible nitric oxide synthase.