Figure 1.

Cytotoxic effects of ³H-Spn alone and combined with SE on HER14 cells. The cells were incubated for 48 h (A) in the presence of varying concentrations of ³H-Spn and (B) with either 1.5 µg/mL ³H-Spn or unlabelled Spn, and varying concentrations of SE applied 5 min after Spn, or with SE alone. Living cells were quantified by their ability to cleave fluorescein diacetate. Relative cell survival was calculated as the number of living cells after treatment in relation to (A) untreated cells and (B) cells treated only with ³H-Spn, Spn without SE or untreated cells. Error bars indicate the SEM of n independent experiments performed in triplicate (n= 4 for ³H-Spn toxicity, n= 3 for ³H-Spn/SE and Spn/SE, and n= 5 for SE). SE, chimeric toxins containing saporin and epidermal growth factor; Spn, Saponinum album from Gypsophila paniculata L.