Figure 3.
Analysis of [3H]-Spn in the urine of mice by HPTLC. Four urine samples from mice without tumours collected 5 min, 10 min (two samples) and 60 min after 3H-Spn injection were analysed by HPTLC. The radioactivity of the application spot and of the area where Spn migrated on the silica gel glass plate was analysed by liquid scintillation counting. The error bars on the data represent the variation in the HPTLC assay carried out in triplicate. HPTLC, high-performance, thin-layer chromatography; Spn, Saponinum album from Gypsophila paniculata L.