Table 4.
Expression levels of mRNA in brown adipose tissue (BAT), white adipose tissue (WAT) and liver from MCH1 receptor antagonist-treated or pair-fed DIO mice
| Tissue/mRNA | Vehicle/ad lib.-fed | MCH1 antagonist | Vehicle/pair-fed |
|---|---|---|---|
| BAT | |||
| UCP1 | 0.79 ± 0.11 | 1.31 ± 0.15** | 1.26 ± 0.12* |
| UCP2 | 0.84 ± 0.04 | 0.78 ± 0.06 | 0.84 ± 0.06 |
| UCP3 | 0.81 ± 0.16 | 1.50 ± 0.28* | 1.42 ± 0.14* |
| CPT1M | 0.66 ± 0.14 | 1.26 ± 0.14** | 1.02 ± 0.08 |
| WAT | |||
| UCP1 | 0.68 ± 0.31 | 1.53 ± 0.69 | 1.19 ± 0.72 |
| UCP2 | 0.55 ± 0.05 | 0.72 ± 0.08 | 0.66 ± 0.03 |
| UCP3 | 1.13 ± 0.30 | 2.31 ± 0.29** | 1.53 ± 0.17 |
| β3AR | 0.72 ± 0.12 | 1.21 ± 0.13** | 0.97 ± 0.09 |
| SREBP1c | 1.01 ± 0.17 | 1.73 ± 0.19**,# | 0.98 ± 0.09 |
| FAS | 0.90 ± 0.19 | 1.96 ± 0.26**,# | 1.21 ± 0.11 |
| GLUT4 | 2.45 ± 0.18 | 3.61 ± 0.31** | 2.79 ± 0.19 |
| Liver | |||
| SREBP1c | 1.03 ± 0.05 | 0.82 ± 0.07* | 0.71 ± 0.06** |
| FAS | 1.32 ± 0.21 | 0.90 ± 0.10* | 0.84 ± 0.12* |
| CPT1L | 1.39 ± 0.09 | 1.32 ± 0.11 | 1.42 ± 0.16 |
Data are means ± standard error (n= 10–13).
*
P < 0.05;
**
P < 0.01 versus vehicle/ad lib-fed group;
#
P < 0.01 versus vehicle/pair-fed group.
β3AR, β3-adrenoceptor; CPT1L, liver carnitine palmitoyltransferase 1; CPT1M, muscle carnitine palmitoyltransferase 1; FAS, fatty acid synthase; GLUT4, glucose transporter 4; SREBP1, sterol-regulatory element-binding protein-1; UCP, uncoupling protein.