Figure 1.

Effect of amiloride (Ami) on the expression of IRE1α and endoplasmic reticulum stress-induced IRE1α phosphorylation. (A) Cells were treated with Ami (200–500 µM) for 1 h and then stimulated with tunicamycin (Tm: 0.01 µg·mL⁻¹) or dithiothreitol (DTT: 3 mM) for 5 h. Western blotting was performed using anti-IRE1α antibody. The band was detected at about 130 kDa∼ for normal and shifted band for IRE1α. Amiloride inhibited Tm-induced IRE1α phosphorylation. Amiloride inhibited the expression of IRE1α. (B) Densitometric analysis of total IRE1α expression using image analyzing software. **P < 0.01 versus control (n= 5). Cont, control.